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pcre sacb zeo murphy  (Addgene inc)


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    Addgene inc pcre sacb zeo murphy
    Pcre Sacb Zeo Murphy, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcre+sacb+zeo+murphy/pKM461+(Plasmid+%23108320)/pm41385366-328-137-135
    Average 93 stars, based on 11 article reviews
    pcre sacb zeo murphy - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Mycobacteria trehalose dimycolate interactions with host Mincle remodel blood-brain barrier junctions for brain invasion.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER mincle sequencing R primer sequence: GCTATGCTCTTGAATAAGATGTGC This paper N/A MSMEG_1531del ultramer: GGGTTCGATGCTGCCCCCCGGTTTCTCCGCCGAGA GGTGCACGTCAGTGACCAAGCAGCCCGGAAAGCTA CAACCAGGTTTGTCTGGTCAACCACCGCGGTCTC AGTGGTGTACGGTACAAACCATCGACGTCAACCA GTTCACCCTGGCGAAGTAGCGGCTCTTCAGAT TCGCTATGCGGGACCTGCCCCGACACGATA This paper N/A JS285 (add lox66/71 to pKM464 F primer sequence): GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGA TCATTACCGTTCGTATAGCATACATTATACGAAGTT ATCTCGAGTCTAGAGCATGCACTAGT This paper N/A JS286 (add lox66/71 to pKM464 R primer sequence): TACCGTTCGTATAATGTATGCTATACGAAGT TATAAGCTTATCGATGTCGACGTAGT This paper N/A JS360 (amplify pknD Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCACGCCCTGTACG This paper N/A JS358 (amplify vector for GGA F primer sequence): GGCTACGGTCTCTCAAAGGCGGTAATACGGTTATC This paper N/A JS359 (amplify vector for GGA R primer sequence): GGCTACGGTCTCATCGTGATACGCCTATTTTTATAG This paper N/A JS361 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTCACTAGTTCTAGAGAACGACCG AGTGGTGAAAC This paper N/A JS362 (amplify hygR for GGA F primer sequence): GGCTACGGTCTCTAGTGGATCCATAACTTCG This paper N/A JS363 (amplify hygR for GGA R primer sequence): GGCTACGGTCTCATTCCGCAGGTAGGGTCGC TCGAGGGTACCGGCGCG This paper N/A JS364 (amplify pknD Mtb upstream R primer sequence): GGCTACGGTCTCAGGAACGGCATCGCTCACC This paper N/A JS365 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTTTTGGATATCCACAGATCCAAGCCC This paper N/A JS372 (ΔpknD M. tuberculosis sequencing R primer sequence): GGTGGTGTTTCGTCCGCTTAC This paper N/A JS373 (ΔpknD M. tuberculosis sequencing F primer sequence): ATGGAGCAGTTCGTCTATGCCTAC This paper N/A JS444 (amplify pcaA Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCGGTGCCTTCGAAGCATTC This paper N/A JS445 (amplify pcaA Mtb downstream F primer sequence): GGCTACGGTCTCGCACTACCGACGTCGACCAGTTCACA This paper N/A JS446 (amplify pcaA Mtb upstream R primer sequence): GGCTACGGTCTCCGGAATCCAAAATGCGGCGTGAGCTG This paper N/A JS447 (amplify pcaA Mtb upstream F primer sequence): GGCTACGGTCTCTTTTGGATATCGACAAGATCGGTTACGACG This paper N/A JS448 (MSMEG_1351 deletion sequencing F primer sequence): ATCTTCTCCAGCAACAACCA This paper N/A JS449 (MSMEG_1351 deletion sequencing R primer sequence): ACCTTGGACAGCTTTTCGATG This paper N/A JS465 (ΔpcaA M. tuberculosis R primer sequence): AGACCCCACGATGGCTTACAC This paper N/A JS466 (ΔpcaA M. tuberculosis sequencing F primer sequence): GCACATCGCCAACAACCTTAT This paper N/A (Continued on next page) Cell Reports 44, 116661, December 23, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER JS485 (linearise pMV261 F primer sequence): CATTGCGAAGTGATTCCTCC This paper N/A JS486 (linearise pMV261 R primer sequence): TAGCGTACGATCGACTGC This paper N/A JS487 (amplify pknD Mm F primer sequence): TCCCCGATCCGGAGGAATCACTTCGCAA TGGTGAGCGAGACCGGGCCG This paper N/A JS488 (amplify pknD Mm R primer sequence): ATTTGATGCCTGGCAGTCGATCGTACGCTA TCAGGATCCTTGGGCCAGTTTCAG This paper N/A JS493 (linearise pTEC27 F primer sequence): CACTTTCTGGCTGGATGATG This paper N/A JS494 (linearise pTEC27 R primer sequence): CCACGGTTGATGAGAGCT This paper N/A JS495 (amplify hsp60:pknD Mm F primer sequence): CACCTACAACAAAGCTCTCATCAACCGTGGAAA TCTAGACGGTGACCACAACG This paper N/A JS496 (amplify hsp60:pknD Mm R primer sequence): TGAATCGCCCCATCATCCAGCCAGAAAGT GTTGTTGGCTAGCTGATCACC This paper N/A Recombinant DNA pTEC18 (msp12:eBFP2) Takaki et al., 2013 72 Addgene #30177 pTEC27 (msp12:tdTomato;hygR) Takaki et al., 2013 72 Addgene #30182 pTEC31 (msp12:tdTomato;kanR) Conrad et al., 2017 46 N/A pNitET-SacB-Kan Murphy et al., 2015 79 Addgene #107692 pKM461 Murphy et al., 2018 80 Addgene #108320 pCre-SacB-Zeo Murphy et al., 2015 79 Addgene #107706 pJKS146 (pKM464 [Addgene #108322] modified to have lox66/71 sites flanking attB) This paper N/A pJKS181 (pKM342 [Addgene #71486] with inserts to delete pknD) This paper N/A pJKS217 (pKM342 [Addgene #71486] with inserts to delete pcaA) This paper N/A pJKS226 (msp12:tdTomato;hsp60:pknD Mm ) This paper N/A gRNAs Mincle gRNA 1: GGTGAAGAGAAGCGTAACTT This paper N/A Mincle gRNA 2: GAGATCTGTGACACTCAAAT This paper N/A Mincle gRNA 3: AGCCTAACATGGGTCTGCAG This paper N/A Software and algorithms Imaris Bitplane https://imaris.oxinst.com/ Adobe Illustrator Adobe https://www.adobe.com/ products/illustrator.html Prism GraphPad https://www.graphpad.com/ ImageJ ImageJ https://imagej.net/ FPC (ImageJ); macro for quantification of bacterial burden by fluorescence imaging Takaki et al., 2013 72 N/A Zen Black Zeiss N/A 22 Cell Reports 44, 116661, December 23, 2025 ..

    Recombinant:

    Article Title: Mycobacteria trehalose dimycolate interactions with host Mincle remodel blood-brain barrier junctions for brain invasion.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER mincle sequencing R primer sequence: GCTATGCTCTTGAATAAGATGTGC This paper N/A MSMEG_1531del ultramer: GGGTTCGATGCTGCCCCCCGGTTTCTCCGCCGAGA GGTGCACGTCAGTGACCAAGCAGCCCGGAAAGCTA CAACCAGGTTTGTCTGGTCAACCACCGCGGTCTC AGTGGTGTACGGTACAAACCATCGACGTCAACCA GTTCACCCTGGCGAAGTAGCGGCTCTTCAGAT TCGCTATGCGGGACCTGCCCCGACACGATA This paper N/A JS285 (add lox66/71 to pKM464 F primer sequence): GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGA TCATTACCGTTCGTATAGCATACATTATACGAAGTT ATCTCGAGTCTAGAGCATGCACTAGT This paper N/A JS286 (add lox66/71 to pKM464 R primer sequence): TACCGTTCGTATAATGTATGCTATACGAAGT TATAAGCTTATCGATGTCGACGTAGT This paper N/A JS360 (amplify pknD Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCACGCCCTGTACG This paper N/A JS358 (amplify vector for GGA F primer sequence): GGCTACGGTCTCTCAAAGGCGGTAATACGGTTATC This paper N/A JS359 (amplify vector for GGA R primer sequence): GGCTACGGTCTCATCGTGATACGCCTATTTTTATAG This paper N/A JS361 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTCACTAGTTCTAGAGAACGACCG AGTGGTGAAAC This paper N/A JS362 (amplify hygR for GGA F primer sequence): GGCTACGGTCTCTAGTGGATCCATAACTTCG This paper N/A JS363 (amplify hygR for GGA R primer sequence): GGCTACGGTCTCATTCCGCAGGTAGGGTCGC TCGAGGGTACCGGCGCG This paper N/A JS364 (amplify pknD Mtb upstream R primer sequence): GGCTACGGTCTCAGGAACGGCATCGCTCACC This paper N/A JS365 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTTTTGGATATCCACAGATCCAAGCCC This paper N/A JS372 (ΔpknD M. tuberculosis sequencing R primer sequence): GGTGGTGTTTCGTCCGCTTAC This paper N/A JS373 (ΔpknD M. tuberculosis sequencing F primer sequence): ATGGAGCAGTTCGTCTATGCCTAC This paper N/A JS444 (amplify pcaA Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCGGTGCCTTCGAAGCATTC This paper N/A JS445 (amplify pcaA Mtb downstream F primer sequence): GGCTACGGTCTCGCACTACCGACGTCGACCAGTTCACA This paper N/A JS446 (amplify pcaA Mtb upstream R primer sequence): GGCTACGGTCTCCGGAATCCAAAATGCGGCGTGAGCTG This paper N/A JS447 (amplify pcaA Mtb upstream F primer sequence): GGCTACGGTCTCTTTTGGATATCGACAAGATCGGTTACGACG This paper N/A JS448 (MSMEG_1351 deletion sequencing F primer sequence): ATCTTCTCCAGCAACAACCA This paper N/A JS449 (MSMEG_1351 deletion sequencing R primer sequence): ACCTTGGACAGCTTTTCGATG This paper N/A JS465 (ΔpcaA M. tuberculosis R primer sequence): AGACCCCACGATGGCTTACAC This paper N/A JS466 (ΔpcaA M. tuberculosis sequencing F primer sequence): GCACATCGCCAACAACCTTAT This paper N/A (Continued on next page) Cell Reports 44, 116661, December 23, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER JS485 (linearise pMV261 F primer sequence): CATTGCGAAGTGATTCCTCC This paper N/A JS486 (linearise pMV261 R primer sequence): TAGCGTACGATCGACTGC This paper N/A JS487 (amplify pknD Mm F primer sequence): TCCCCGATCCGGAGGAATCACTTCGCAA TGGTGAGCGAGACCGGGCCG This paper N/A JS488 (amplify pknD Mm R primer sequence): ATTTGATGCCTGGCAGTCGATCGTACGCTA TCAGGATCCTTGGGCCAGTTTCAG This paper N/A JS493 (linearise pTEC27 F primer sequence): CACTTTCTGGCTGGATGATG This paper N/A JS494 (linearise pTEC27 R primer sequence): CCACGGTTGATGAGAGCT This paper N/A JS495 (amplify hsp60:pknD Mm F primer sequence): CACCTACAACAAAGCTCTCATCAACCGTGGAAA TCTAGACGGTGACCACAACG This paper N/A JS496 (amplify hsp60:pknD Mm R primer sequence): TGAATCGCCCCATCATCCAGCCAGAAAGT GTTGTTGGCTAGCTGATCACC This paper N/A Recombinant DNA pTEC18 (msp12:eBFP2) Takaki et al., 2013 72 Addgene #30177 pTEC27 (msp12:tdTomato;hygR) Takaki et al., 2013 72 Addgene #30182 pTEC31 (msp12:tdTomato;kanR) Conrad et al., 2017 46 N/A pNitET-SacB-Kan Murphy et al., 2015 79 Addgene #107692 pKM461 Murphy et al., 2018 80 Addgene #108320 pCre-SacB-Zeo Murphy et al., 2015 79 Addgene #107706 pJKS146 (pKM464 [Addgene #108322] modified to have lox66/71 sites flanking attB) This paper N/A pJKS181 (pKM342 [Addgene #71486] with inserts to delete pknD) This paper N/A pJKS217 (pKM342 [Addgene #71486] with inserts to delete pcaA) This paper N/A pJKS226 (msp12:tdTomato;hsp60:pknD Mm ) This paper N/A gRNAs Mincle gRNA 1: GGTGAAGAGAAGCGTAACTT This paper N/A Mincle gRNA 2: GAGATCTGTGACACTCAAAT This paper N/A Mincle gRNA 3: AGCCTAACATGGGTCTGCAG This paper N/A Software and algorithms Imaris Bitplane https://imaris.oxinst.com/ Adobe Illustrator Adobe https://www.adobe.com/ products/illustrator.html Prism GraphPad https://www.graphpad.com/ ImageJ ImageJ https://imagej.net/ FPC (ImageJ); macro for quantification of bacterial burden by fluorescence imaging Takaki et al., 2013 72 N/A Zen Black Zeiss N/A 22 Cell Reports 44, 116661, December 23, 2025 ..

    Modification:

    Article Title: Mycobacteria trehalose dimycolate interactions with host Mincle remodel blood-brain barrier junctions for brain invasion.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER mincle sequencing R primer sequence: GCTATGCTCTTGAATAAGATGTGC This paper N/A MSMEG_1531del ultramer: GGGTTCGATGCTGCCCCCCGGTTTCTCCGCCGAGA GGTGCACGTCAGTGACCAAGCAGCCCGGAAAGCTA CAACCAGGTTTGTCTGGTCAACCACCGCGGTCTC AGTGGTGTACGGTACAAACCATCGACGTCAACCA GTTCACCCTGGCGAAGTAGCGGCTCTTCAGAT TCGCTATGCGGGACCTGCCCCGACACGATA This paper N/A JS285 (add lox66/71 to pKM464 F primer sequence): GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGA TCATTACCGTTCGTATAGCATACATTATACGAAGTT ATCTCGAGTCTAGAGCATGCACTAGT This paper N/A JS286 (add lox66/71 to pKM464 R primer sequence): TACCGTTCGTATAATGTATGCTATACGAAGT TATAAGCTTATCGATGTCGACGTAGT This paper N/A JS360 (amplify pknD Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCACGCCCTGTACG This paper N/A JS358 (amplify vector for GGA F primer sequence): GGCTACGGTCTCTCAAAGGCGGTAATACGGTTATC This paper N/A JS359 (amplify vector for GGA R primer sequence): GGCTACGGTCTCATCGTGATACGCCTATTTTTATAG This paper N/A JS361 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTCACTAGTTCTAGAGAACGACCG AGTGGTGAAAC This paper N/A JS362 (amplify hygR for GGA F primer sequence): GGCTACGGTCTCTAGTGGATCCATAACTTCG This paper N/A JS363 (amplify hygR for GGA R primer sequence): GGCTACGGTCTCATTCCGCAGGTAGGGTCGC TCGAGGGTACCGGCGCG This paper N/A JS364 (amplify pknD Mtb upstream R primer sequence): GGCTACGGTCTCAGGAACGGCATCGCTCACC This paper N/A JS365 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTTTTGGATATCCACAGATCCAAGCCC This paper N/A JS372 (ΔpknD M. tuberculosis sequencing R primer sequence): GGTGGTGTTTCGTCCGCTTAC This paper N/A JS373 (ΔpknD M. tuberculosis sequencing F primer sequence): ATGGAGCAGTTCGTCTATGCCTAC This paper N/A JS444 (amplify pcaA Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCGGTGCCTTCGAAGCATTC This paper N/A JS445 (amplify pcaA Mtb downstream F primer sequence): GGCTACGGTCTCGCACTACCGACGTCGACCAGTTCACA This paper N/A JS446 (amplify pcaA Mtb upstream R primer sequence): GGCTACGGTCTCCGGAATCCAAAATGCGGCGTGAGCTG This paper N/A JS447 (amplify pcaA Mtb upstream F primer sequence): GGCTACGGTCTCTTTTGGATATCGACAAGATCGGTTACGACG This paper N/A JS448 (MSMEG_1351 deletion sequencing F primer sequence): ATCTTCTCCAGCAACAACCA This paper N/A JS449 (MSMEG_1351 deletion sequencing R primer sequence): ACCTTGGACAGCTTTTCGATG This paper N/A JS465 (ΔpcaA M. tuberculosis R primer sequence): AGACCCCACGATGGCTTACAC This paper N/A JS466 (ΔpcaA M. tuberculosis sequencing F primer sequence): GCACATCGCCAACAACCTTAT This paper N/A (Continued on next page) Cell Reports 44, 116661, December 23, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER JS485 (linearise pMV261 F primer sequence): CATTGCGAAGTGATTCCTCC This paper N/A JS486 (linearise pMV261 R primer sequence): TAGCGTACGATCGACTGC This paper N/A JS487 (amplify pknD Mm F primer sequence): TCCCCGATCCGGAGGAATCACTTCGCAA TGGTGAGCGAGACCGGGCCG This paper N/A JS488 (amplify pknD Mm R primer sequence): ATTTGATGCCTGGCAGTCGATCGTACGCTA TCAGGATCCTTGGGCCAGTTTCAG This paper N/A JS493 (linearise pTEC27 F primer sequence): CACTTTCTGGCTGGATGATG This paper N/A JS494 (linearise pTEC27 R primer sequence): CCACGGTTGATGAGAGCT This paper N/A JS495 (amplify hsp60:pknD Mm F primer sequence): CACCTACAACAAAGCTCTCATCAACCGTGGAAA TCTAGACGGTGACCACAACG This paper N/A JS496 (amplify hsp60:pknD Mm R primer sequence): TGAATCGCCCCATCATCCAGCCAGAAAGT GTTGTTGGCTAGCTGATCACC This paper N/A Recombinant DNA pTEC18 (msp12:eBFP2) Takaki et al., 2013 72 Addgene #30177 pTEC27 (msp12:tdTomato;hygR) Takaki et al., 2013 72 Addgene #30182 pTEC31 (msp12:tdTomato;kanR) Conrad et al., 2017 46 N/A pNitET-SacB-Kan Murphy et al., 2015 79 Addgene #107692 pKM461 Murphy et al., 2018 80 Addgene #108320 pCre-SacB-Zeo Murphy et al., 2015 79 Addgene #107706 pJKS146 (pKM464 [Addgene #108322] modified to have lox66/71 sites flanking attB) This paper N/A pJKS181 (pKM342 [Addgene #71486] with inserts to delete pknD) This paper N/A pJKS217 (pKM342 [Addgene #71486] with inserts to delete pcaA) This paper N/A pJKS226 (msp12:tdTomato;hsp60:pknD Mm ) This paper N/A gRNAs Mincle gRNA 1: GGTGAAGAGAAGCGTAACTT This paper N/A Mincle gRNA 2: GAGATCTGTGACACTCAAAT This paper N/A Mincle gRNA 3: AGCCTAACATGGGTCTGCAG This paper N/A Software and algorithms Imaris Bitplane https://imaris.oxinst.com/ Adobe Illustrator Adobe https://www.adobe.com/ products/illustrator.html Prism GraphPad https://www.graphpad.com/ ImageJ ImageJ https://imagej.net/ FPC (ImageJ); macro for quantification of bacterial burden by fluorescence imaging Takaki et al., 2013 72 N/A Zen Black Zeiss N/A 22 Cell Reports 44, 116661, December 23, 2025 ..

    Software:

    Article Title: Mycobacteria trehalose dimycolate interactions with host Mincle remodel blood-brain barrier junctions for brain invasion.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER mincle sequencing R primer sequence: GCTATGCTCTTGAATAAGATGTGC This paper N/A MSMEG_1531del ultramer: GGGTTCGATGCTGCCCCCCGGTTTCTCCGCCGAGA GGTGCACGTCAGTGACCAAGCAGCCCGGAAAGCTA CAACCAGGTTTGTCTGGTCAACCACCGCGGTCTC AGTGGTGTACGGTACAAACCATCGACGTCAACCA GTTCACCCTGGCGAAGTAGCGGCTCTTCAGAT TCGCTATGCGGGACCTGCCCCGACACGATA This paper N/A JS285 (add lox66/71 to pKM464 F primer sequence): GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGA TCATTACCGTTCGTATAGCATACATTATACGAAGTT ATCTCGAGTCTAGAGCATGCACTAGT This paper N/A JS286 (add lox66/71 to pKM464 R primer sequence): TACCGTTCGTATAATGTATGCTATACGAAGT TATAAGCTTATCGATGTCGACGTAGT This paper N/A JS360 (amplify pknD Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCACGCCCTGTACG This paper N/A JS358 (amplify vector for GGA F primer sequence): GGCTACGGTCTCTCAAAGGCGGTAATACGGTTATC This paper N/A JS359 (amplify vector for GGA R primer sequence): GGCTACGGTCTCATCGTGATACGCCTATTTTTATAG This paper N/A JS361 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTCACTAGTTCTAGAGAACGACCG AGTGGTGAAAC This paper N/A JS362 (amplify hygR for GGA F primer sequence): GGCTACGGTCTCTAGTGGATCCATAACTTCG This paper N/A JS363 (amplify hygR for GGA R primer sequence): GGCTACGGTCTCATTCCGCAGGTAGGGTCGC TCGAGGGTACCGGCGCG This paper N/A JS364 (amplify pknD Mtb upstream R primer sequence): GGCTACGGTCTCAGGAACGGCATCGCTCACC This paper N/A JS365 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTTTTGGATATCCACAGATCCAAGCCC This paper N/A JS372 (ΔpknD M. tuberculosis sequencing R primer sequence): GGTGGTGTTTCGTCCGCTTAC This paper N/A JS373 (ΔpknD M. tuberculosis sequencing F primer sequence): ATGGAGCAGTTCGTCTATGCCTAC This paper N/A JS444 (amplify pcaA Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCGGTGCCTTCGAAGCATTC This paper N/A JS445 (amplify pcaA Mtb downstream F primer sequence): GGCTACGGTCTCGCACTACCGACGTCGACCAGTTCACA This paper N/A JS446 (amplify pcaA Mtb upstream R primer sequence): GGCTACGGTCTCCGGAATCCAAAATGCGGCGTGAGCTG This paper N/A JS447 (amplify pcaA Mtb upstream F primer sequence): GGCTACGGTCTCTTTTGGATATCGACAAGATCGGTTACGACG This paper N/A JS448 (MSMEG_1351 deletion sequencing F primer sequence): ATCTTCTCCAGCAACAACCA This paper N/A JS449 (MSMEG_1351 deletion sequencing R primer sequence): ACCTTGGACAGCTTTTCGATG This paper N/A JS465 (ΔpcaA M. tuberculosis R primer sequence): AGACCCCACGATGGCTTACAC This paper N/A JS466 (ΔpcaA M. tuberculosis sequencing F primer sequence): GCACATCGCCAACAACCTTAT This paper N/A (Continued on next page) Cell Reports 44, 116661, December 23, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER JS485 (linearise pMV261 F primer sequence): CATTGCGAAGTGATTCCTCC This paper N/A JS486 (linearise pMV261 R primer sequence): TAGCGTACGATCGACTGC This paper N/A JS487 (amplify pknD Mm F primer sequence): TCCCCGATCCGGAGGAATCACTTCGCAA TGGTGAGCGAGACCGGGCCG This paper N/A JS488 (amplify pknD Mm R primer sequence): ATTTGATGCCTGGCAGTCGATCGTACGCTA TCAGGATCCTTGGGCCAGTTTCAG This paper N/A JS493 (linearise pTEC27 F primer sequence): CACTTTCTGGCTGGATGATG This paper N/A JS494 (linearise pTEC27 R primer sequence): CCACGGTTGATGAGAGCT This paper N/A JS495 (amplify hsp60:pknD Mm F primer sequence): CACCTACAACAAAGCTCTCATCAACCGTGGAAA TCTAGACGGTGACCACAACG This paper N/A JS496 (amplify hsp60:pknD Mm R primer sequence): TGAATCGCCCCATCATCCAGCCAGAAAGT GTTGTTGGCTAGCTGATCACC This paper N/A Recombinant DNA pTEC18 (msp12:eBFP2) Takaki et al., 2013 72 Addgene #30177 pTEC27 (msp12:tdTomato;hygR) Takaki et al., 2013 72 Addgene #30182 pTEC31 (msp12:tdTomato;kanR) Conrad et al., 2017 46 N/A pNitET-SacB-Kan Murphy et al., 2015 79 Addgene #107692 pKM461 Murphy et al., 2018 80 Addgene #108320 pCre-SacB-Zeo Murphy et al., 2015 79 Addgene #107706 pJKS146 (pKM464 [Addgene #108322] modified to have lox66/71 sites flanking attB) This paper N/A pJKS181 (pKM342 [Addgene #71486] with inserts to delete pknD) This paper N/A pJKS217 (pKM342 [Addgene #71486] with inserts to delete pcaA) This paper N/A pJKS226 (msp12:tdTomato;hsp60:pknD Mm ) This paper N/A gRNAs Mincle gRNA 1: GGTGAAGAGAAGCGTAACTT This paper N/A Mincle gRNA 2: GAGATCTGTGACACTCAAAT This paper N/A Mincle gRNA 3: AGCCTAACATGGGTCTGCAG This paper N/A Software and algorithms Imaris Bitplane https://imaris.oxinst.com/ Adobe Illustrator Adobe https://www.adobe.com/ products/illustrator.html Prism GraphPad https://www.graphpad.com/ ImageJ ImageJ https://imagej.net/ FPC (ImageJ); macro for quantification of bacterial burden by fluorescence imaging Takaki et al., 2013 72 N/A Zen Black Zeiss N/A 22 Cell Reports 44, 116661, December 23, 2025 ..

    Fluorescence:

    Article Title: Mycobacteria trehalose dimycolate interactions with host Mincle remodel blood-brain barrier junctions for brain invasion.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER mincle sequencing R primer sequence: GCTATGCTCTTGAATAAGATGTGC This paper N/A MSMEG_1531del ultramer: GGGTTCGATGCTGCCCCCCGGTTTCTCCGCCGAGA GGTGCACGTCAGTGACCAAGCAGCCCGGAAAGCTA CAACCAGGTTTGTCTGGTCAACCACCGCGGTCTC AGTGGTGTACGGTACAAACCATCGACGTCAACCA GTTCACCCTGGCGAAGTAGCGGCTCTTCAGAT TCGCTATGCGGGACCTGCCCCGACACGATA This paper N/A JS285 (add lox66/71 to pKM464 F primer sequence): GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGA TCATTACCGTTCGTATAGCATACATTATACGAAGTT ATCTCGAGTCTAGAGCATGCACTAGT This paper N/A JS286 (add lox66/71 to pKM464 R primer sequence): TACCGTTCGTATAATGTATGCTATACGAAGT TATAAGCTTATCGATGTCGACGTAGT This paper N/A JS360 (amplify pknD Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCACGCCCTGTACG This paper N/A JS358 (amplify vector for GGA F primer sequence): GGCTACGGTCTCTCAAAGGCGGTAATACGGTTATC This paper N/A JS359 (amplify vector for GGA R primer sequence): GGCTACGGTCTCATCGTGATACGCCTATTTTTATAG This paper N/A JS361 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTCACTAGTTCTAGAGAACGACCG AGTGGTGAAAC This paper N/A JS362 (amplify hygR for GGA F primer sequence): GGCTACGGTCTCTAGTGGATCCATAACTTCG This paper N/A JS363 (amplify hygR for GGA R primer sequence): GGCTACGGTCTCATTCCGCAGGTAGGGTCGC TCGAGGGTACCGGCGCG This paper N/A JS364 (amplify pknD Mtb upstream R primer sequence): GGCTACGGTCTCAGGAACGGCATCGCTCACC This paper N/A JS365 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTTTTGGATATCCACAGATCCAAGCCC This paper N/A JS372 (ΔpknD M. tuberculosis sequencing R primer sequence): GGTGGTGTTTCGTCCGCTTAC This paper N/A JS373 (ΔpknD M. tuberculosis sequencing F primer sequence): ATGGAGCAGTTCGTCTATGCCTAC This paper N/A JS444 (amplify pcaA Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCGGTGCCTTCGAAGCATTC This paper N/A JS445 (amplify pcaA Mtb downstream F primer sequence): GGCTACGGTCTCGCACTACCGACGTCGACCAGTTCACA This paper N/A JS446 (amplify pcaA Mtb upstream R primer sequence): GGCTACGGTCTCCGGAATCCAAAATGCGGCGTGAGCTG This paper N/A JS447 (amplify pcaA Mtb upstream F primer sequence): GGCTACGGTCTCTTTTGGATATCGACAAGATCGGTTACGACG This paper N/A JS448 (MSMEG_1351 deletion sequencing F primer sequence): ATCTTCTCCAGCAACAACCA This paper N/A JS449 (MSMEG_1351 deletion sequencing R primer sequence): ACCTTGGACAGCTTTTCGATG This paper N/A JS465 (ΔpcaA M. tuberculosis R primer sequence): AGACCCCACGATGGCTTACAC This paper N/A JS466 (ΔpcaA M. tuberculosis sequencing F primer sequence): GCACATCGCCAACAACCTTAT This paper N/A (Continued on next page) Cell Reports 44, 116661, December 23, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER JS485 (linearise pMV261 F primer sequence): CATTGCGAAGTGATTCCTCC This paper N/A JS486 (linearise pMV261 R primer sequence): TAGCGTACGATCGACTGC This paper N/A JS487 (amplify pknD Mm F primer sequence): TCCCCGATCCGGAGGAATCACTTCGCAA TGGTGAGCGAGACCGGGCCG This paper N/A JS488 (amplify pknD Mm R primer sequence): ATTTGATGCCTGGCAGTCGATCGTACGCTA TCAGGATCCTTGGGCCAGTTTCAG This paper N/A JS493 (linearise pTEC27 F primer sequence): CACTTTCTGGCTGGATGATG This paper N/A JS494 (linearise pTEC27 R primer sequence): CCACGGTTGATGAGAGCT This paper N/A JS495 (amplify hsp60:pknD Mm F primer sequence): CACCTACAACAAAGCTCTCATCAACCGTGGAAA TCTAGACGGTGACCACAACG This paper N/A JS496 (amplify hsp60:pknD Mm R primer sequence): TGAATCGCCCCATCATCCAGCCAGAAAGT GTTGTTGGCTAGCTGATCACC This paper N/A Recombinant DNA pTEC18 (msp12:eBFP2) Takaki et al., 2013 72 Addgene #30177 pTEC27 (msp12:tdTomato;hygR) Takaki et al., 2013 72 Addgene #30182 pTEC31 (msp12:tdTomato;kanR) Conrad et al., 2017 46 N/A pNitET-SacB-Kan Murphy et al., 2015 79 Addgene #107692 pKM461 Murphy et al., 2018 80 Addgene #108320 pCre-SacB-Zeo Murphy et al., 2015 79 Addgene #107706 pJKS146 (pKM464 [Addgene #108322] modified to have lox66/71 sites flanking attB) This paper N/A pJKS181 (pKM342 [Addgene #71486] with inserts to delete pknD) This paper N/A pJKS217 (pKM342 [Addgene #71486] with inserts to delete pcaA) This paper N/A pJKS226 (msp12:tdTomato;hsp60:pknD Mm ) This paper N/A gRNAs Mincle gRNA 1: GGTGAAGAGAAGCGTAACTT This paper N/A Mincle gRNA 2: GAGATCTGTGACACTCAAAT This paper N/A Mincle gRNA 3: AGCCTAACATGGGTCTGCAG This paper N/A Software and algorithms Imaris Bitplane https://imaris.oxinst.com/ Adobe Illustrator Adobe https://www.adobe.com/ products/illustrator.html Prism GraphPad https://www.graphpad.com/ ImageJ ImageJ https://imagej.net/ FPC (ImageJ); macro for quantification of bacterial burden by fluorescence imaging Takaki et al., 2013 72 N/A Zen Black Zeiss N/A 22 Cell Reports 44, 116661, December 23, 2025 ..

    Imaging:

    Article Title: Mycobacteria trehalose dimycolate interactions with host Mincle remodel blood-brain barrier junctions for brain invasion.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER mincle sequencing R primer sequence: GCTATGCTCTTGAATAAGATGTGC This paper N/A MSMEG_1531del ultramer: GGGTTCGATGCTGCCCCCCGGTTTCTCCGCCGAGA GGTGCACGTCAGTGACCAAGCAGCCCGGAAAGCTA CAACCAGGTTTGTCTGGTCAACCACCGCGGTCTC AGTGGTGTACGGTACAAACCATCGACGTCAACCA GTTCACCCTGGCGAAGTAGCGGCTCTTCAGAT TCGCTATGCGGGACCTGCCCCGACACGATA This paper N/A JS285 (add lox66/71 to pKM464 F primer sequence): GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGA TCATTACCGTTCGTATAGCATACATTATACGAAGTT ATCTCGAGTCTAGAGCATGCACTAGT This paper N/A JS286 (add lox66/71 to pKM464 R primer sequence): TACCGTTCGTATAATGTATGCTATACGAAGT TATAAGCTTATCGATGTCGACGTAGT This paper N/A JS360 (amplify pknD Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCACGCCCTGTACG This paper N/A JS358 (amplify vector for GGA F primer sequence): GGCTACGGTCTCTCAAAGGCGGTAATACGGTTATC This paper N/A JS359 (amplify vector for GGA R primer sequence): GGCTACGGTCTCATCGTGATACGCCTATTTTTATAG This paper N/A JS361 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTCACTAGTTCTAGAGAACGACCG AGTGGTGAAAC This paper N/A JS362 (amplify hygR for GGA F primer sequence): GGCTACGGTCTCTAGTGGATCCATAACTTCG This paper N/A JS363 (amplify hygR for GGA R primer sequence): GGCTACGGTCTCATTCCGCAGGTAGGGTCGC TCGAGGGTACCGGCGCG This paper N/A JS364 (amplify pknD Mtb upstream R primer sequence): GGCTACGGTCTCAGGAACGGCATCGCTCACC This paper N/A JS365 (amplify pknD Mtb downstream F primer sequence): GGCTACGGTCTCTTTTGGATATCCACAGATCCAAGCCC This paper N/A JS372 (ΔpknD M. tuberculosis sequencing R primer sequence): GGTGGTGTTTCGTCCGCTTAC This paper N/A JS373 (ΔpknD M. tuberculosis sequencing F primer sequence): ATGGAGCAGTTCGTCTATGCCTAC This paper N/A JS444 (amplify pcaA Mtb downstream R primer sequence): GGCTACGGTCTCAACGAGATATCGGTGCCTTCGAAGCATTC This paper N/A JS445 (amplify pcaA Mtb downstream F primer sequence): GGCTACGGTCTCGCACTACCGACGTCGACCAGTTCACA This paper N/A JS446 (amplify pcaA Mtb upstream R primer sequence): GGCTACGGTCTCCGGAATCCAAAATGCGGCGTGAGCTG This paper N/A JS447 (amplify pcaA Mtb upstream F primer sequence): GGCTACGGTCTCTTTTGGATATCGACAAGATCGGTTACGACG This paper N/A JS448 (MSMEG_1351 deletion sequencing F primer sequence): ATCTTCTCCAGCAACAACCA This paper N/A JS449 (MSMEG_1351 deletion sequencing R primer sequence): ACCTTGGACAGCTTTTCGATG This paper N/A JS465 (ΔpcaA M. tuberculosis R primer sequence): AGACCCCACGATGGCTTACAC This paper N/A JS466 (ΔpcaA M. tuberculosis sequencing F primer sequence): GCACATCGCCAACAACCTTAT This paper N/A (Continued on next page) Cell Reports 44, 116661, December 23, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER JS485 (linearise pMV261 F primer sequence): CATTGCGAAGTGATTCCTCC This paper N/A JS486 (linearise pMV261 R primer sequence): TAGCGTACGATCGACTGC This paper N/A JS487 (amplify pknD Mm F primer sequence): TCCCCGATCCGGAGGAATCACTTCGCAA TGGTGAGCGAGACCGGGCCG This paper N/A JS488 (amplify pknD Mm R primer sequence): ATTTGATGCCTGGCAGTCGATCGTACGCTA TCAGGATCCTTGGGCCAGTTTCAG This paper N/A JS493 (linearise pTEC27 F primer sequence): CACTTTCTGGCTGGATGATG This paper N/A JS494 (linearise pTEC27 R primer sequence): CCACGGTTGATGAGAGCT This paper N/A JS495 (amplify hsp60:pknD Mm F primer sequence): CACCTACAACAAAGCTCTCATCAACCGTGGAAA TCTAGACGGTGACCACAACG This paper N/A JS496 (amplify hsp60:pknD Mm R primer sequence): TGAATCGCCCCATCATCCAGCCAGAAAGT GTTGTTGGCTAGCTGATCACC This paper N/A Recombinant DNA pTEC18 (msp12:eBFP2) Takaki et al., 2013 72 Addgene #30177 pTEC27 (msp12:tdTomato;hygR) Takaki et al., 2013 72 Addgene #30182 pTEC31 (msp12:tdTomato;kanR) Conrad et al., 2017 46 N/A pNitET-SacB-Kan Murphy et al., 2015 79 Addgene #107692 pKM461 Murphy et al., 2018 80 Addgene #108320 pCre-SacB-Zeo Murphy et al., 2015 79 Addgene #107706 pJKS146 (pKM464 [Addgene #108322] modified to have lox66/71 sites flanking attB) This paper N/A pJKS181 (pKM342 [Addgene #71486] with inserts to delete pknD) This paper N/A pJKS217 (pKM342 [Addgene #71486] with inserts to delete pcaA) This paper N/A pJKS226 (msp12:tdTomato;hsp60:pknD Mm ) This paper N/A gRNAs Mincle gRNA 1: GGTGAAGAGAAGCGTAACTT This paper N/A Mincle gRNA 2: GAGATCTGTGACACTCAAAT This paper N/A Mincle gRNA 3: AGCCTAACATGGGTCTGCAG This paper N/A Software and algorithms Imaris Bitplane https://imaris.oxinst.com/ Adobe Illustrator Adobe https://www.adobe.com/ products/illustrator.html Prism GraphPad https://www.graphpad.com/ ImageJ ImageJ https://imagej.net/ FPC (ImageJ); macro for quantification of bacterial burden by fluorescence imaging Takaki et al., 2013 72 N/A Zen Black Zeiss N/A 22 Cell Reports 44, 116661, December 23, 2025 ..



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